Enzyme-linked Immunosorbent Assay:Article Title: Hemoglobin Binders Reduce Inflammation and Tissue Injury from Hemolysis in Venovenous Extracorporeal Circulation.
Article Snippet: 551 Analyte Assay Type Vendor Kit H-Ferritin ELISA Abcam, Cambridge, UK ab108837# L-Ferritin ELISA LSBio, Shirley, MA LS-F7873-1* S100B ELISA Elabscience, Houston, TX E-EL-R0868* HIF-1α ELISA Elabscience, Houston, TX E-EL-R0513* NF-κB ELISA Abcam, Cambridge, UK ab176663$# Fractalkine ELISA Abcam, Cambridge, UK ab100760* IL-1β ELISA Thermo Fisher, Waltham, MA BMS630* Creatinine Colorimetric Arbor Assays Inc, Ann Arbor, MI KB02-H2 BUN Colorimetric Arbor Assays Inc, Ann Arbor, MI K024-H5 NGAL ELISA Thermo Fisher, Waltham, MA ERLCN2* 4-HNE ELISA Abcam, Cambridge, UK ab287803 KIM-1 ELISA Abcam, Cambridge, UK ab223858* HO-1 ELISA Abcam, Cambridge, UK ab279414* Syndecan-1 ELISA Novus Biologicals, Centennial, CO NBP2-76611* Fibrinogen ELISA Abcam, Cambridge, UK ab324756@ Catecholamines (epi, norepi) ELISA ImmunoSmol, Bordeaux, France BA-E-6600 IL-6 ELISA Thermo Fisher, Waltham, MA BMS625* IL-10 ELISA Thermo Fisher, Waltham, MA BMS629* Neut Flow Cytometry BD Biosciences, Franklin Lakes, NJ 554875* MPO ELISA Abcam, Cambridge, UK ab285308* FABP2 ELISA LSBio, Shirley, MA LS-F5611-1$ FABP6 ELISA LSBio, Shirley, MA LS-F19585-1* Bilirubin Colorimetric Abcam, Cambridge, UK ab235627 AST Colorimetric Abnova Corp, Taiwan KA1625 ALT Colorimetric Abnova Corp, Taiwan KA4189 ANP ELISA Sigma-Aldrich, St. Louis, MO RAB0385*$# Troponin ELISA Abcam, Cambridge, UK ab246529* TNF-α ELISA Abcam, Cambridge, UK ab100785* 552 D ow nloaded from http://ashpublications.org/bloodadvances/article-pdf/doi/10.1182/bloodadvances.2025017619/2500300/bloodadvances.2025017619.pdf by guest on 27 January 2026 28 Table 4.
Flow Cytometry:Article Title: Hemoglobin Binders Reduce Inflammation and Tissue Injury from Hemolysis in Venovenous Extracorporeal Circulation.
Article Snippet: 551 Analyte Assay Type Vendor Kit H-Ferritin ELISA Abcam, Cambridge, UK ab108837# L-Ferritin ELISA LSBio, Shirley, MA LS-F7873-1* S100B ELISA Elabscience, Houston, TX E-EL-R0868* HIF-1α ELISA Elabscience, Houston, TX E-EL-R0513* NF-κB ELISA Abcam, Cambridge, UK ab176663$# Fractalkine ELISA Abcam, Cambridge, UK ab100760* IL-1β ELISA Thermo Fisher, Waltham, MA BMS630* Creatinine Colorimetric Arbor Assays Inc, Ann Arbor, MI KB02-H2 BUN Colorimetric Arbor Assays Inc, Ann Arbor, MI K024-H5 NGAL ELISA Thermo Fisher, Waltham, MA ERLCN2* 4-HNE ELISA Abcam, Cambridge, UK ab287803 KIM-1 ELISA Abcam, Cambridge, UK ab223858* HO-1 ELISA Abcam, Cambridge, UK ab279414* Syndecan-1 ELISA Novus Biologicals, Centennial, CO NBP2-76611* Fibrinogen ELISA Abcam, Cambridge, UK ab324756@ Catecholamines (epi, norepi) ELISA ImmunoSmol, Bordeaux, France BA-E-6600 IL-6 ELISA Thermo Fisher, Waltham, MA BMS625* IL-10 ELISA Thermo Fisher, Waltham, MA BMS629* Neut Flow Cytometry BD Biosciences, Franklin Lakes, NJ 554875* MPO ELISA Abcam, Cambridge, UK ab285308* FABP2 ELISA LSBio, Shirley, MA LS-F5611-1$ FABP6 ELISA LSBio, Shirley, MA LS-F19585-1* Bilirubin Colorimetric Abcam, Cambridge, UK ab235627 AST Colorimetric Abnova Corp, Taiwan KA1625 ALT Colorimetric Abnova Corp, Taiwan KA4189 ANP ELISA Sigma-Aldrich, St. Louis, MO RAB0385*$# Troponin ELISA Abcam, Cambridge, UK ab246529* TNF-α ELISA Abcam, Cambridge, UK ab100785* 552 D ow nloaded from http://ashpublications.org/bloodadvances/article-pdf/doi/10.1182/bloodadvances.2025017619/2500300/bloodadvances.2025017619.pdf by guest on 27 January 2026 28 Table 4.
AST Assay:Article Title: Hemoglobin Binders Reduce Inflammation and Tissue Injury from Hemolysis in Venovenous Extracorporeal Circulation.
Article Snippet: 551 Analyte Assay Type Vendor Kit H-Ferritin ELISA Abcam, Cambridge, UK ab108837# L-Ferritin ELISA LSBio, Shirley, MA LS-F7873-1* S100B ELISA Elabscience, Houston, TX E-EL-R0868* HIF-1α ELISA Elabscience, Houston, TX E-EL-R0513* NF-κB ELISA Abcam, Cambridge, UK ab176663$# Fractalkine ELISA Abcam, Cambridge, UK ab100760* IL-1β ELISA Thermo Fisher, Waltham, MA BMS630* Creatinine Colorimetric Arbor Assays Inc, Ann Arbor, MI KB02-H2 BUN Colorimetric Arbor Assays Inc, Ann Arbor, MI K024-H5 NGAL ELISA Thermo Fisher, Waltham, MA ERLCN2* 4-HNE ELISA Abcam, Cambridge, UK ab287803 KIM-1 ELISA Abcam, Cambridge, UK ab223858* HO-1 ELISA Abcam, Cambridge, UK ab279414* Syndecan-1 ELISA Novus Biologicals, Centennial, CO NBP2-76611* Fibrinogen ELISA Abcam, Cambridge, UK ab324756@ Catecholamines (epi, norepi) ELISA ImmunoSmol, Bordeaux, France BA-E-6600 IL-6 ELISA Thermo Fisher, Waltham, MA BMS625* IL-10 ELISA Thermo Fisher, Waltham, MA BMS629* Neut Flow Cytometry BD Biosciences, Franklin Lakes, NJ 554875* MPO ELISA Abcam, Cambridge, UK ab285308* FABP2 ELISA LSBio, Shirley, MA LS-F5611-1$ FABP6 ELISA LSBio, Shirley, MA LS-F19585-1* Bilirubin Colorimetric Abcam, Cambridge, UK ab235627 AST Colorimetric Abnova Corp, Taiwan KA1625 ALT Colorimetric Abnova Corp, Taiwan KA4189 ANP ELISA Sigma-Aldrich, St. Louis, MO RAB0385*$# Troponin ELISA Abcam, Cambridge, UK ab246529* TNF-α ELISA Abcam, Cambridge, UK ab100785* 552 D ow nloaded from http://ashpublications.org/bloodadvances/article-pdf/doi/10.1182/bloodadvances.2025017619/2500300/bloodadvances.2025017619.pdf by guest on 27 January 2026 28 Table 4.
Aqueous Normal-phase Chromatography:Article Title: Hemoglobin Binders Reduce Inflammation and Tissue Injury from Hemolysis in Venovenous Extracorporeal Circulation.
Article Snippet: 551 Analyte Assay Type Vendor Kit H-Ferritin ELISA Abcam, Cambridge, UK ab108837# L-Ferritin ELISA LSBio, Shirley, MA LS-F7873-1* S100B ELISA Elabscience, Houston, TX E-EL-R0868* HIF-1α ELISA Elabscience, Houston, TX E-EL-R0513* NF-κB ELISA Abcam, Cambridge, UK ab176663$# Fractalkine ELISA Abcam, Cambridge, UK ab100760* IL-1β ELISA Thermo Fisher, Waltham, MA BMS630* Creatinine Colorimetric Arbor Assays Inc, Ann Arbor, MI KB02-H2 BUN Colorimetric Arbor Assays Inc, Ann Arbor, MI K024-H5 NGAL ELISA Thermo Fisher, Waltham, MA ERLCN2* 4-HNE ELISA Abcam, Cambridge, UK ab287803 KIM-1 ELISA Abcam, Cambridge, UK ab223858* HO-1 ELISA Abcam, Cambridge, UK ab279414* Syndecan-1 ELISA Novus Biologicals, Centennial, CO NBP2-76611* Fibrinogen ELISA Abcam, Cambridge, UK ab324756@ Catecholamines (epi, norepi) ELISA ImmunoSmol, Bordeaux, France BA-E-6600 IL-6 ELISA Thermo Fisher, Waltham, MA BMS625* IL-10 ELISA Thermo Fisher, Waltham, MA BMS629* Neut Flow Cytometry BD Biosciences, Franklin Lakes, NJ 554875* MPO ELISA Abcam, Cambridge, UK ab285308* FABP2 ELISA LSBio, Shirley, MA LS-F5611-1$ FABP6 ELISA LSBio, Shirley, MA LS-F19585-1* Bilirubin Colorimetric Abcam, Cambridge, UK ab235627 AST Colorimetric Abnova Corp, Taiwan KA1625 ALT Colorimetric Abnova Corp, Taiwan KA4189 ANP ELISA Sigma-Aldrich, St. Louis, MO RAB0385*$# Troponin ELISA Abcam, Cambridge, UK ab246529* TNF-α ELISA Abcam, Cambridge, UK ab100785* 552 D ow nloaded from http://ashpublications.org/bloodadvances/article-pdf/doi/10.1182/bloodadvances.2025017619/2500300/bloodadvances.2025017619.pdf by guest on 27 January 2026 28 Table 4.
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